Mycobacterium tuberculosis stimulated gamma interferon and spot count panel - Blood 74281-7
LOINC Code
| LOINC code | 74281-7 | ||
|---|---|---|---|
| name | Mycobacterium tuberculosis stimulated gamma interferon and spot count panel - Blood | ||
| description | Interferon gamma release assays (IGRAs) are used for the detection of M. tuberculosis. This panel was created for, but not limited in use to, Oxford Immunotec's T-SPOT.TB assay, which uses enzyme-linked immunospot (ELISPOT) methodology to enumerate M. tuberculosis-sensitized T cells by capturing interferon-gamma (IFN-y) in the vicinity of T cells in the patient's whole blood. Cells are spun and, using the ficoll centrifugation procedure, the polys and RBCs layer together in the bottom fraction with peripheral blood mononuclear cells (PBMCs) in the middle. The PBMCs are pipetted out to yield about 250,000 PBMCs per aliquot. They are incubated with M. tuberculosis specific antigens (ESAT-6, CFP10) in two separate wells, a phytohemagglutinin (PHA) control (a mitogenic stimulator) in another well, and a negative control in a fourth well. Incubation with the antigens allows for stimulation of any sensitized T cells present. Evaluating the number of antigen incubated spots compared to the positive and negative control spots provides a measurement of the abundance of M. tuberculosis sensitive T cells in the patient's blood. | ||
| status | ACTIVE | ||
Fully-Specified Name | |||
| component | Mycobacterium tuberculosis stimulated gamma interferon & spot count panel | ||
| property | - | ||
| time | Pt = Point in time: To identify measures at a point in time. This is a synonym for “spot” or “random” as applied to urine measurements. | ||
| system | Bld = Whole blood | ||
| scale | - | ||
| method | |||
Additional Names | |||
| short name | M TB IFN-g+spot Pnl Bld | ||
Basic Attributes | |||
| class | PANEL.MICRO | ||
| type | 1 Laboratory | ||
| order vs. observation | Order | ||
Panel Components | |||
Associated Observations | |||
LOINC codes that represent optional associated observation(s) for a clinical observation or laboratory test. A LOINC term may represent a single associated observation or panel containing several associated observations. | |||
History/Usage | |||
| first released | |||
| last updated | 2.64 | ||
| last change type | MIN - change to field other than name | ||
Related Names | |||
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