Purine/Pyrimidine [Ratio] in Red Blood Cells by Spectrophotometry 85379-6
LOINC Code
| LOINC code | 85379-6 | ||
|---|---|---|---|
| name | Purine/Pyrimidine [Ratio] in Red Blood Cells by Spectrophotometry | ||
| description | The purine to pyrimidine ratio is a marker of pyrimidine-5-nucleotidase (P5N) which is an enzyme in the cytoplasm of human red blood cells. The function of P5N is unknown but a deficiency of the enzyme causes congenital haemolytic anemia. Individuals who are heterozygous for the recessive P5N gene demonstrate no clinical or hematological abnormalities while those homozygous for the recessive P5N gene have a corresponding 5-30% reduction in normal enzyme activity. P5N deficiency leads to chronic non-spherocytic haemolytic anaemia associated with mild to moderate hemolysis, increased basophilic stippling in 5% of the red blood cells and an elevation of red cell glutathione and pyrimidine nucleotides. The diagnosis of P5N deficiency is based on the change in the level of cytidine and uridine in red blood cells which is normally very low but increases to over 50% in P5N deficient patients. Measurement of P5N is by light absorption at different wavelengths. In acidic solution pyrimidine nucleotides absorb the maximum amount of light at 280 nanometers (nm). Purine nucleotides absorb the maximum amount of light at 260 nm. The ratio of light absorbed at 260 nm to that at 280 nm in a pool of red blood cells indicates the relative amount of purines to pyrimidines. The ratio is lower than normal when pyrimidine levels are increased.[PMID: 23992312][ Dacie and Lewis Practical Haematology, 11th edition, Barbara J. Bain, Imelda Bates, Mike A. Laffan, S. Mitchell Lewis, p259] | ||
| status | ACTIVE | ||
Fully-Specified Name | |||
| component | Purine/pyrimidine | ||
| property | Ratio | ||
| time | Pt = Point in time: To identify measures at a point in time. This is a synonym for “spot” or “random” as applied to urine measurements. | ||
| system | RBC = Erythrocytes | ||
| scale | Qn = Quantitative: The result of the test is a numeric value that relates to a continuous numeric scale. Reported either as an integer, a ratio, a real number, or a range. The test result value may optionally contain a relational operator from the set {<=, <, >, >=}. Valid values for a quantitative test are of the form "7", "-7", "7.4", "-7.4", "7.8912", "0.125", "<10", "<10.15", ">12000", 1-10, 1:256 | ||
| method | Spectrophotometry | ||
Additional Names | |||
| short name | Purine/Pyrimidine RBC Spect-Rto | ||
Basic Attributes | |||
| class | CHEM | ||
| type | 1 Laboratory | ||
| order vs. observation | Both | ||
| example UCUM units | {ratio} The Unified Code for Units of Measure (UCUM) is a code system intended to include all units of measures being contemporarily used in international science, engineering, and business (www.unitsofmeasure.org) | ||
| example unit | ratio | ||
Associated Observations | |||
LOINC codes that represent optional associated observation(s) for a clinical observation or laboratory test. A LOINC term may represent a single associated observation or panel containing several associated observations. | |||
History/Usage | |||
| first released | |||
| last updated | 2.61 | ||
| last change type | ADD - added | ||
Related Names | |||
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